Description

stat summarizes opt binding predictions

Input

name:type
description
pattern

meta

:map

Groovy Map containing information of the probe targets generated from the panel sequences with opt track e.g. [ id:'breast_cancer_probe_panel_sequences' ]

probe_targets

:file

Generated probe targets

*.tsv

meta2

:map

Groovy Map containing information of the forward oriented fasta generated with the probes panel sequences ‘opt flip’ e.g. [ id:'breast_cancer_probe_panel_sequences' ]

fwd_oriented_probes

:file

The forward oriented fasta file

*.fa

gene_synonyms

:file

Gene synonyms that may have been counted as off-targets but simply differ in name (optional input)

*.csv

Output

name:type
description
pattern

summary

meta

:map

Groovy Map containing summary of the forward oriented probes generated with the panel sequences ‘opt flip and track’ e.g. [ id:'breast_cancer_probe_panel_sequences' ]

${meta.id}/collapsed_summary.tsv

:file

tsv file containing the summary stats

*.tsv

versions

versions.yml

:file

File containing software versions

versions.yml

Tools

opt
GPL-3.0 license

opt is a simple program that aligns probe sequences to transcript sequences to detect potential off-target probe activity